The three generations of sequencing

Authors

  • Rocío Bautista Moreno Spain

Keywords:

three generations, sequencing

Abstract

"When Sanger, with the enzymatic chain termination method (dideoxynucleotide method), and Gilbert, with the chemical fragmentation method, developed the first approaches to DNA sequencing in the 70s, neither of them could imagine the speed at which this process would evolve thirty years later."

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References

Sanger, F., Nicklen S., Coulson, AR. DNA sequencing with chain-terminating inhibitors. Proc Natl. Acad. Sci. USA, 74: 5463-5467 (1977)

Mostafa Ronaghi, Samer Karamohamed, Bertil Pet- tersson, Mathias Uhlén and Pål Nyrén. Real-Time DNA Sequencing Using Detection of Pyrophosphate Re- lease. Analytical Biochemistry. 242: 84-89 (1996) Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science. 269:496-512 (1995)

Published

2010-05-20

How to Cite

Bautista Moreno, R. (2010). The three generations of sequencing. Encuentros En La Biología, 3(128), 27–28. Retrieved from https://revistas.uma.es/index.php/enbio/article/view/18518