In search of ‘N0’: following the yellow brick road"

Authors

  • Jan M. Rujiter Spain
  • Adrián Ruiz Villalba Spain

Keywords:

transcriptase, reverse, gene expression

Abstract

In the last 15 years, the quantification of the reverse transcriptase chain reaction (RT-qPCR) has become the most widely used method for the quantification of gene expression (Baker M, Nature Methods, 2011, 8(3): 207-212). Some of its characteristics have contributed to the widespread use of this methodology: i) it is a one-step assay, without the need for post-PCR processing; ii) it allows a simple comparison between transcripts with a wide range of expression (differences 107 times); and iii) it uses the quantitative potential implicit in a conventional PCR, making it both a quantitative and qualitative assay.

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Published

2013-07-20

How to Cite

M. Rujiter, J., & Ruiz Villalba , A. (2013). In search of ‘N0’: following the yellow brick road". Encuentros En La Biología, 6(143), 47–50. Retrieved from https://revistas.uma.es/index.php/enbio/article/view/18375