Capture by laser microdissection, revealing the secrets of a cell

Authors

  • Rafael A. Cañas Spain
  • Fernando de la Torre Spain

Keywords:

laser microdissection, cell

Abstract

Laser microdissection capture is a powerful microscopy technique that allows the isolation and capture of tissues or individual cells from histological sections or cell cultures. Since the beginning of the 20th century, the bases that led to its final development in 1996 by Emmert-Buck and later marketed by Arcturus. This technique, mainly developed as a tool for pathologists, has spread to different branches of medicine and biology and is an established method in molecular biology, neuroscience, developmental biology, cancer research, forensic medicine, proteomics, metabolomics or plant research

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References

Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA. Láser capture microdissection.

Science 274:998-1001, 1996

Página web del sistema LCM de Arcturus. http://www.lifetechnologies.com/es/en/home/life-science/gene-expression-analysis genotyping/láser-capture-microdissection.html

Página web del sistema LCM de Leica en Leica Science Lab. http://www.leica-microsystems.com/science-lab/topics/láser-microdissection/

Cañas RA, Canales J, Gómez-Maldonado J, Avila C, Cánovas FM. Transcriptome analysis in maritime pine using láser capture microdissection and 454 pyrosequencing. Tree Physiology. doi: 10.1093/treephys/tpt113, 2014

Published

2015-03-21

How to Cite

Cañas , R. A., & de la Torre, F. (2015). Capture by laser microdissection, revealing the secrets of a cell. Encuentros En La Biología, 8(154), 61–63. Retrieved from https://revistas.uma.es/index.php/enbio/article/view/18110

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Artículos