Echinospartum algibicum (Leguminosae), plant regeneration via adventitious organogenesis
DOI:
https://doi.org/10.24310/abm.v22i0.8604Keywords:
Conservation, Eehinospartum, seed germination, mycorrhizaAbstract
In vitro seed germination of E. algibicum, an endangered species, multiple bud induction and shoot growth was achieved on a growth medium composed of half strength Murashige and Skoog medium, with 3% sucrose and BAP at 1 and 2 mg (ECH- I , ECH-2). Seed germination increased significantly after scarification (from 38% to almost 100%). The mean number of buds formed after 35-40 days was 8.6 and 6.9 on ECH-1 and ECH-2 media respectively. Shoots were then rooted on the same medium but substituting the cytokinin by the auxin IBA (0.2 mg 1 -1 ). On this medium the mean maximum rooting percentage was 35.49 after 25-30 days; in some cases, callus formation took place on the shoot areas in contact with the nutrient medium. Finally, acclimatization of rooted shoots was achieved through progressive reduction of relative humidity and after several treatments including use of CO2, addition of mycorrhizal fungus (Glomus deserticola) inocula to the substrate, as well as the use of a nutrient medium lacking any plant growth regulators with half the amount of sucrose, prior transferring to soil. After 60 days in soil, survival rates were higher for plants treated with CO2 , although the mean survival percentages were rather low.
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