Limonium ×erbenii (Plumbaginaceae), new hybrid taxon for the Southeastern Iberian flora

Auteurs

DOI :

https://doi.org/10.24310/abm.51.2026.22810

Mots-clés :

interspecific hybrid, morphometrics, new nothospecies, nrDNA, sea lavanders, Taxonomy

Résumé

Limonium ×erbenii, a hybrid resulting from the interspecific cross between Limonium estevei Fern. Casas and Limonium cossonianum Kuntze, is here described as a new nothospecies based on the morphological and molecular characterization of five individuals collected in Macenas, at the base of Sierra de Cabrera (Mojácar, Almería, SE Spain), where it coexists with the parental taxa. The morphological analysis included the measurement and comparison of 39 diagnostic characters to evaluate the intermediacy of the hybrid accessions relative to the parental species. For the molecular characterization, the ITS region of ribosomal nrDNA was amplified, sequenced and analyzed to detect potential additive polymorphic sites (APS). Results revealed a mosaic of parental and intermediate morphological traits, a typical pattern of a first-generation hybrid (F1), and the presence of APS indicative of the coexistence of ribotypes inherited from both parental species within the hybrid’s genetic background. Altogether, these findings provide strong evidence confirming Limonium ×erbenii as the product of natural hybridization between L. cossonianum and L. estevei.

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Références

Aguilar, J. F., & Nieto-Feliner, G. (2003). Additive polymorphisms and reticulation in an ITS phylogeny of thrifts (Armeria, Plumbaginaceae). Molecular Phylogenetics and Evolution, 28(3), 430-447. doi: https://doi.org/10.1016/S1055-7903(02)00301-9

Aguilar, J. F., Rosselló, J. A., & Nieto-Feliner, G. (1999). Nuclear ribosomal DNA (nrDNA) concerted evolution in natural and artificial hybrids of Armeria (Plumbaginaceae). Molecular Ecology, 8(8), 1341-1346. doi: https://doi.org/10.1046/j.1365-294X.1999.00690.x

Álvarez, I., & Wendel, J. F. (2003). Ribosomal ITS sequences and plant phylogenetic inference. Molecular phylogenetics and evolution, 29(3), 417-434. doi: https://doi.org/10.1016/S1055-7903(03)00208-2

Baldwin, B. G., Sanderson, M. J., Porter, J. M., Wojciechowski, M. F., Campbell, C. S., & Donoghue, M. J. (1995). The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Annals of the Missouri Botanical Garden, 82(2) 247-277. doi: https://doi.org/10.2307/2399880

Chase, M. W., & Hills, H. H. (1991). Silica gel: an ideal material for field preservation of leaf samples for DNA studies. Taxon, 40(2), 215-220. doi: https://doi.org/10.2307/1222975

Erben, M. (1978). Die gattung Limonium im südwestmediterranen Raum. In H. Merxmüller (Ed.), Mitteilungen der Botanischen Staatssammlung München (Vol. 14, pp. 361–631). Munich: Botanischen Staatssammlung München.

Erben, M. (1993). Limonium Miller. In S. Castroviejo, C. Aedo, S. Cirujano, M. Laínz, P. Montserrat, R. Morales, F. Muñoz-Garmendia, C. Navarro, J. Paiva, & C. Soriano (Eds.), Flora iberica (Vol. 3, pp. 2-143). Madrid: Real Jardín Botánico-C.S.I.C.

Farell, E. M., & Alexandre, G. (2012). Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates. BMC research notes, 5(1), 257. doi: https://doi.org/10.1186/1756-0500-5-257

Fernández-Casas, J., & García-Villaraco, A. (1979). Números cromosomáticos de plantas occidentales, 38-45. Anales del Jardin Botánico de Madrid, 36, 395-398.

García-Rodríguez, R., de Vega, C., Schwarzer, H., Rubio-Pérez, E., Martín-Hernanz, S., Fernández-Mazuecos, M., Blanco-Pastor, J. L., Aparicio, A., & Albaladejo, R. G. (2026). Conservation genomics of the critically endangered sea lavender Limonium estevei: a recent bottleneck and low introgression from a widespread congener. Conservation Genetics, 27(1), 12. https://doi.org/10.1007/s10592-025-01741-y

Hammer, Ø., Harper, D. A. T, & Ryan, P. D. (2001). PAST: Paleontological Statistics software package for education and data analysis. Palaeontologia Electronica 4(1), 1–9. doi: http://palaeo-electronica.org/2001_1/past/issue1_01.htm

Iamonico, D., De Castro, O., Di Iorio, E., Nicolella, G., & Iberite, M. (2022). Taxonomy Complexity of Some Tyrrhenian Endemic Limonium Species Belonging to L. multiforme Group (Plumbaginaceae): New Insights from Molecular and Morphometric Analyses. Plants, 11(22), 3163. doi: https://doi.org/10.3390/plants11223163

Kubitzki, K. (1993). Plumbaginaceae. In: K. Kubitzki, J. G. Rohwer, & V. Bittrich (Eds.), Flowering Plants · Dicotyledons. The Families and Genera of Vascular Plants, Vol. II. (pp. 523-530). Heidelberg, Berlin: Springer. doi: https://doi.org/10.1007/978-3-662-02899-5_62

Miranda-Hernández, L., Salmerón-Sánchez, E., Fernández-Cobo, M. J., Merlo, E., Salazar-Mendías, C., Mendoza-Fernández, A. J., Martínez-Hernández, F., Pérez-García, F. J., & Mota, J. F. (2026). Hybridization, ecological niche, and conservation of the threatened endemic species Limonium estevei: an integrated approach in the genus Limonium. 35, 54. doi: https://doi.org/10.1007/s10531-026-03254-7.

Nieto-Feliner, G., Gutiérrez-Larena, B., & Fuertes-Aguilar, J. (2004). Fine‐scale geographical structure, intra‐individual polymorphism and recombination in nuclear ribosomal internal transcribed spacers in Armeria (Plumbaginaceae). Annals of Botany, 93(2), 189-200. doi: https://doi.org/10.1093/aob/mch027

Palacios, C., Rosselló, J. A., & González-Candelas, F. (2000). Study of the evolutionary relationships among Limonium species (Plumbaginaceae) using nuclear and cytoplasmic molecular markers. Molecular Phylogenetics and Evolution, 14(2), 232-249. doi: https://doi.org/10.1006/mpev.1999.0690

Plants of the World Online. (2024). Limonium Mill. Electronic resource in https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:331722-2. Accessed on 8 August 2025.

Rieseberg, L. H., Ellstrand, N. C., & Arnold, M. (1993). What can molecular and morphological markers tell us about plant hybridization? Critical Reviews in Plant Sciences, 12(3), 213-241. doi: https://doi.org/10.1080/07352689309701902

Salmerón-Sánchez, E., Pérez-García, F. J., Medina-Cazorla, J. M., Martínez-Nieto, M. I., Martínez-Hernández, F., Garrido-Becerra, J. A., Mendoza-Fernández, A. J., Merlo, E., & Mota, J. F. (2014). Genetic analysis based on plastidial and ribosomal sequences of the endemic bi-edaphic taxon Jurinea pinnata (Lag.) DC.(Compositae) in the Guadix-Baza Basin. Plant Biosystems, 149(5), 922-932. doi: https://doi.org/10.1080/11263504.2014.983203

Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7), 671–675. doi: https://doi.org/10.1038/nmeth.2089

Thiers, B. M. (continuously updated). Index Herbariorum: A global directory of public herbaria and associated staff. Electronic resource in https://sweetgum.nybg.org/science/ih/

Turland, N. J., Wiersema, J. H., Barrie, F. R., Gandhi, K. N., Gravendyck, J., Greuter, W., Hawksworth, D. L., Herendeen, P. S., Klopper, R. R., Knapp, S., Kusberg, W., Li, D., May, T. W., Monro, A. M., Prado, J., Price, M. J., Smith, G. F., & Zamora, J. C. (2025). International Code of Nomenclature for algae, fungi, and plants (Madrid Code). Chicago: University of Chicago Press. doi: https://doi.org/10.7208/chicago/9780226839479.001.0001

Comparativa flores y semillas de L. estevei, L. ×erbenii y L. cossonianum

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Publiée

2026-02-13

Comment citer

Limonium ×erbenii (Plumbaginaceae), new hybrid taxon for the Southeastern Iberian flora. (2026). Acta Botanica Malacitana, 51. https://doi.org/10.24310/abm.51.2026.22810

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Limonium ×erbenii (Plumbaginaceae), new hybrid taxon for the Southeastern Iberian flora. (2026). Acta Botanica Malacitana, 51. https://doi.org/10.24310/abm.51.2026.22810

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